Tag PCR

In analyzing the plasmid sequence from the whole genome sequences of AHPND V. parahaemolyticus (V.p) isolates, researchers identified a clear geographical variation within the plasmid, and developed PCR methods to characterize AHPND V.p isolates as either Mexico-type or Southeast Asia-type. 

A study confirmed the utility for improved polymerase chain reaction (PCR) detection of the Vibrio bacteria that cause acute hepatopancreatic necrosis disease (AHPND) in asymptomatic shrimp by permitting the shrimp to expire and decompose for several hours prior to preservation and PCR processing. 

Real-time, quantitative polymerase chain reaction (qPCR) analysis has become an attractive alternative for quantification of the acute hepatopancreatic necrosis disease (AHPND) pathogen in infected shrimp. The authors developed a qPCR assay to quantify a virulence plasmid harbored by the bacterium that causes AHPND. 

Polymerase chain reaction testing is a powerful tool that can detect very low levels of pathogens, but its ability to screen and produce results that represent the population is often oversimplified. Screening of the wrong tissues or too few animals can lead to incorrect conclusions. 

Offering high sensitivity and specificity, polymerase chain reaction (PCR) assays are the most accepted molecular method for white spot syndrome virus (WSSV) detection. 

Establishing postlarvae quality has become one of the most important aspects in controlling diseases in farmed shrimp. Initial microbial testing can ensure viruses and other pathogens are absent from the animals. 

The Queiroz Galvão Alimentos shrimp farm and hatchery in Brazil have been working with Concepto Azul to implement a disease-prevention and genetic-breeding program that addresses ongoing impacts from infectious myonecrosis virus (IMNV) and other pathogens. 

As research on WSSV continues, commonsense steps can lessen the potential impacts of white spot syndrome virus on shrimp-farming operations. 

Necrotizing hepatopancreatitis is a disease of shrimp that causes slow growth, low survival and poor feed conversion. Wet mount analysis is commonly used to diagnose NHP in ponds in Brazil. 

The University of Arizona’s Aquaculture Pathology Laboratory oversees ring tests for a group of shrimp diagnostic laboratories in 10 countries. 

A rapid PCR assay for detection of WSSV was based on the nested, two-step PCR procedure recommended in the Manual of Diagnostic Tests for Aquatic Animals. 

Mexico's government, shrimp industry and academic community are working to develop strategies to attenuate the effects of white spot virus in Sinaloa. They confirmed eight genotypes. 

A rapid lateral-flow immunoassay using monoclonal antibody combinations can identify necrotizing hepatopancreatitis (NHP) infection in less than 15 minutes. 

Use of this method to detect and quantify IHHNV in penaeid shrimp has already revealed new information regarding levels of infection. 

Polymerase chain reaction (PCR) is a molecular biology technique that amplifies the number of copies of a specific region of DNA to produce enough to be tested.