Health & Welfare

Real-time PCR offers sensitivity, specificity in detecting AHPND plasmid

Real-time, quantitative polymerase chain reaction (qPCR) analysis has become an attractive alternative for quantification of the acute hepatopancreatic necrosis disease (AHPND) pathogen in infected shrimp. The authors developed a qPCR assay to quantify a virulence plasmid harbored by the bacterium that causes AHPND.